Journal: PLoS ONE

Article Title: Virotherapy Using Myxoma Virus Prevents Lethal Graft-versus-Host Disease following Xeno-Transplantation with Primary Human Hematopoietic Stem Cells

doi: 10.1371/journal.pone.0043298

Figure Lengend Snippet: To determine if MYXV can infect CD3 + lymphocytes found in human HCT samples, 1×10 6 whole BM cells ( A ) or 1×10 6 human PBMCs ( B ) were treated with vMyx-GFP at MOI = 10. Twenty-four hours after MYXV exposure, cells were stained with antibodies against CD3 and CD4 and the levels of GFP + cells in each population was determined using flow cytometry.

Article Snippet: Fresh normal human bone marrow aspirate cells and peripheral blood mononuclear cells were obtained commercially from Lonza (Walkersville, Maryland).

Techniques: Staining, Flow Cytometry

Journal: PLoS ONE

Article Title: Virotherapy Using Myxoma Virus Prevents Lethal Graft-versus-Host Disease following Xeno-Transplantation with Primary Human Hematopoietic Stem Cells

doi: 10.1371/journal.pone.0043298

Figure Lengend Snippet: NSG mice were sublethally irradiated and then transplanted with either 5×10 6 primary human PBMCs (n = 6) or 5×10 6 primary human PBMCs pre-treated with MYXV (n = 6). Mice were weighed twice per week to monitor body condition ( A ) and sacrificed either seven weeks after transplant or when their body condition score measured 2 ( B ). Significant differences in survival were determined using the log-rank test (P<0.05). N.S. = not significant. Post-mortem, organs were extracted, fixed in formalin, sectioned and stained for the presence of human CD3 + lymphocytes ( C ). Immunohistochemistry images shown are representative of results observed in three separate mice from the three engrafted cohorts stained for the presence of human CD3 + cells.

Article Snippet: Fresh normal human bone marrow aspirate cells and peripheral blood mononuclear cells were obtained commercially from Lonza (Walkersville, Maryland).

Techniques: Irradiation, Staining, Immunohistochemistry